Downstream Processing: Chromatography (MBI® Training, Bioprocess Industries)

Teaching is in small classes, with an emphasis on interactive problem solving and case studies.

Each day will cover the following topics:

• Chromatography fundamentals
• Models and scale-up
• Role of adsorption isotherms in chromatography
• Radial flow chromatography

• Pilot-plant studies: chromatography (packing and operation of radial flow column, automation and scale-down for chromatography screening using robotic systems, calculations of productivity and throughput for process chromatography)
• Continuous chromatography in biopharmaceutical industries
• Design and manufacturing of chromatography resins
• Affinity separation
• Process-scale separation with monolith chromatography
• The challenges of large scale chromatography

• Process hygiene and column maintenance
• Case study: mAb purification, considerations for elution and wash-buffers
• The use of chromatography membranes for the development and scale-up of biopharmaceutical processes
• Micro scale-down technologies for high-throughput development of chromatographic separations
• Digital design of chromatograhic bioseparations
• Scale-down and process economic for packed bed bioprocesses
• Nanofibres as flexible, single use alternative to traditional chromatography

• Mixed mode chromatography: understanding interactions and developing purifications
• Design of biomimetics for affinity separations
• Case study: where and when to apply chromatography?
• Where will further improvements in chromatography come from?